Cells, Free Full-Text

Silk has a long history as an exclusive textile, but also as a suture thread in medicine; nowadays, diverse cell carriers are manufactured from silk. Its advantages are manifold, including high biocompatibility, biomechanical strength and processability (approved for nearly all manufacturing techniques). Silk’s limitations, such as scarcity and batch to batch variations, are overcome by gene technology, which allows for the upscaled production of recombinant “designed” silk proteins. For processing thin fibroin filaments, the sericin component is generally removed (degumming). In contrast to many synthetic biomaterials, fibroin allows for superior cell adherence and growth. In addition, silk grafts demonstrate superior mechanical performance and long-term stability, making them attractive for anterior cruciate ligament (ACL) tissue engineering. Looking at these promising properties, this review focusses on the responses of cell types to silk variants, as well as their biomechanical properties, which are relevant for ACL tissue engineering. Meanwhile, sericin has also attracted increasing interest and has been proposed as a bioactive biomaterial with antimicrobial properties. But so far, fibroin was exclusively used for experimental ACL tissue engineering approaches, and fibroin from spider silk also seems not to have been applied. To improve the bone integration of ACL grafts, silk scaffolds with osteogenic functionalization, silk-based tunnel fillers and interference screws have been developed. Nevertheless, signaling pathways stimulated by silk components remain barely elucidated, but need to be considered during the development of optimized silk cell carriers for ACL tissue engineering.

Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect

Cancer type classification using plasma cell-free RNAs derived from human and microbes

Towards reproducible cell-free systems

The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. - Abstract - Europe PMC

Rapid cell-free forward engineering of novel genetic ring oscillators

THE LIVES OF A CELL : LEWIS THOMAS : Free Download, Borrow, and Streaming : Internet Archive

Remote immune processes revealed by immune-derived circulating cell-free DNA

Advances and applications of cell-free systems for metabolic production - ScienceDirect

Iec/Ts 62886 Get File - Colaboratory

Iec/Ts 62886 Get File - Colaboratory

Rapid cell-free forward engineering of novel genetic ring oscillators

Cells, Free Full-Text

HIV-1 cell-to-cell transmission and broadly neutralizing antibodies, Retrovirology

Cell-free gene expression: an expanded repertoire of applications